Ngth (bp) 3862 4438 148,856 Previously, theread high quality the initial data12,six excellent examination showed that the genomic Imply results of 12,7 data ofNumber of reads/contig several base sequences that 351,411increase or affect the error D. aromatica still had could 418,943 1 worth due tolengthread (bp) Study low N50 length and quality. When low read length and high-quality have been re6061 6114 Total bases (bp) moved, the mean read length, mean1,617,953,241 and read length N50 statistically inread high-quality, 1,559,878,347 Average After filtering, around 96 of reads passed the good quality control 186.804 creased (Table 1).coverage (351,411 reads) using a reading length N50 of 6114 bp as well as a total base of 1.55 Gb. The assembly stage in this study was carried out using reference-guided DNA assemTable comparing the raw, filtered, and assembled reads. bly by1. Statistics of thestudied genome using the 17-Hydroxyventuricidin A Purity & Documentation reference genome in bioinformatics analysis. The reference-guided assembly produced a partial genome of D. aromatica chloroplasts of Raw Reads Filtered Reads Assembled Reads 148,856 bp. The GC content was calculated as 36.92 , which can be NSC636819 Histone Demethylase constant with cpDNAs Mean study Dipterocarpaceae household 3862 4438 148,856 from other length/contig length (bp) members, including Hopea reticulata (37.four) [47] and Mean study (37.1) [48]. Several genes with high GC content were exhibited by high-quality 12,6 12,7 Parashorea chinensis Number of reads/contig 418,943 351,411 1 4 ribosomal proteins, namely, rrn23, rrn16, rrn4,5, and rrn5 with 55 , 56 , 50 , and Study length addition, 6061 6114 51 , respectively. InN50 (bp) the total genome fraction identified within the partial genome was Total bases (bp) 1,617,953,241 1,559,878,347 89.99 , with 411 indels and 135,411 alignments for reference. Typical coverage 186.804 Reference assembly is less time-consuming and has computational power [49]. DNA assembly to produce the entire genome begins with combining overlapping reads to construct contigs. The contigsin thiscombined tocarried out utilizing reference-guided DNA asThe assembly stage have been study was make scaffolds, which had been also combined to receive the whole genome. studied genome with all the reference genome in bioinformatics sembly by comparing the Having said that, genome assembly usually meets quite a few challenges (sequencing error, brief reads, repeats, polymorphism, and so on.) that must be resolvedchloanalysis. The reference-guided assembly created a partial genome of D. aromatica and demands of 148,856sequencing before being calculated as 36.92 , which isgenome. Thereroplasts repeated bp. The GC content was capable to construct a total consistent with fore, this from other Dipterocarpaceae family members, including Hopea reticulata (37.4) cpDNAs study focused on the chloroplast genome of D. aromatica as a result of the single sequencing generated in this(37.1) [48]. Quite a few genes with higher GC content material were exhib[47] and Parashorea chinensis study. ited by 4 ribosomal proteins, namely, rrn23, rrn16, rrn4,five, and rrn5 with 55 , 56 , 50 , 3.two. Chloroplast Genome Annotation and 51 , respectively. Moreover, the total genome fraction discovered within the partial genome Genome annotation was performed to identify functional genes along the genome was 89.99 , with 411 indels and 135,411 alignments for reference. sequence [50]. The annotation of D. aromatica chloroplast identifies genes contained in theTable 1. Statistics of your raw, filtered, and assembled reads.(sequencing error, brief reads, repeats, polymorphism, and so forth.).