Ar space. We previously found that extracellular vesicles (EVs) from endothelial progenitor cells (EPCs) stop endothelial dysfunction and lung injury in sepsis on account of their encapsulation of miRNA-126. Nevertheless, the effects of EPC EVs in acute lung injury (ALI) remains unknown. Approaches: To determine if EPC EVs would have useful effects in ALI, intratracheal administration of lipopolysaccharide (LPS) was made use of to induce ALI in mice. Lung permeability, inflammation as well as the part of miRNA-126 in alveolar epithelial barrier function were examined. Results: The intratracheal administration of EPC EVs decreased lung injury following LPS-induced ALI at 24 and 48 h. Compared to placebo, intratracheal administration of EPC EVs substantially reduced the cell number, protein concentration and cytokines/chemokines within the bronchoalveolar lavage fluid, indicating a reduction in permeability and inflammation. Further, EPC EVs lowered myeloperoxidase activity and reduced the lung injury score, demonstrating protection againstIntroduction: Trauma and degeneration of articular cartilage (AC) could trigger the morbidity of among the top disabling disease, osteoarthritis (OA). One of several most difficult issues in therapy would be the poor selfhealing ability of AC. Extracellular vesicle (EV) transplantation has received a lot more and much more consideration as prospective cell-free therapeutic approaches to promote tissue healing. In our preliminary study, we found that decreased expression of hsa_circ_0000077 (circ77) was closely related to OA. And circ77-overexpression in chondrocytes can prevent the chondrocyte degeneration. In this study, EVs derived from circ77-overexpressing synovium mesenchymal stem cells (SMSC-77EVs) have been used to promote cartilage regeneration. Approaches: CCK-8, qPCR and western blotting (WB) were used to investigate the biological functions of SMSC-77-EVs on the proliferation and cartilage regeneration. Furthermore, interleukin 1 (IL-1) were used to simulate the inflammatory circumstances of OA, and then, the protective effects of SMSC-77-EVs have been confirmed by CCK-8, qPCR and WB. Final results: CCK-8 assay confirmed that SMSC-77-EVs enhanced the proliferation of chondrocytes, compared with regular control and EVs derived from synovium mesenchymal stem cells which had been transfected by empty vectors (SMSC-Empty-EVs). WB and qPCR assays confirmed that SMSC-77-EVs enhanced theISEV2019 ABSTRACT BOOKexpression levels of cartilage related proteins including Sort II collagen (Col-II), aggrecan (ACAN) and SOX9, compared with typical control and SMSC-Empty-EVs. IL-1 considerably inhibited the proliferation and cartilage regeneration-related proteins (TLR8 Synonyms Col-II, ACAN and SOX9). SMSC-77-EVs could observably restrain the harmful effects of IL-1, while SMSC-Empty-EVs showed PKCĪ¹ Formulation restricted capacity. Summary/Conclusion: These findings recommend that the novel SMSC-77-EVs gives the preferable function in advertising the repair of cartilage damage. The usage of SMSC-77-EVs would represent a development trend of cell-free therapies, employing engineered EVs (or modularized EVs), for promoting cartilage regeneration. Funding: The National Natural Science Foundation of China [Nos. 81871834, 81802226 and 81301589], and Shanghai Jiao Tong University K.C.Wong Medical Fellowship Fund supported this function.PT12.Lymphangiogenesis induced by exosomes derived from adiposederived mesenchymal stem cells Kensuke Tashiroa, Yusuke Yoshiokab and Takahiro OchiyabaThe incubation time was 48 h in proliferation assa.