M, J. Heald, R. Scratch n9 screen for inhibitors of cell migration. Chem Biol 12, 263 (2005). 27. Huang, C., Rajfur, Z., Borchers, C., Schaller, M. D. Jacobson, K. JNK phosphorylates paxillin and regulates cell migration. Nature 424, 21923 (2003). 28. Lampugnani, M. G. Cell migration into a wounded location in vitro. CDK16 custom synthesis Procedures Mol Biol 96, 1772 (1999). 29. Khan, K. N. M., Paulson, S. K., Verburg, K. M., Lefkowith, J. B. Maziasz, T. J. Pharmacology of cyclooxygenase-2 inhibition within the kidney. Kidney Int 61, 1210 (2002). 30. Guo, X. Nzerue, C. The best way to prevent, recognize, and treat drug-induced nephrotoxicity. Cleve Clin J Med 69, 2890, 293, 296 passim (2002). 31. Harirforoosh, S. Jamali, F. Renal adverse effects of nonsteroidal antiinflammatory drugs. Expert Opin Drug Saf 8, 6691 (2009). 32. Alberts, B. et al. Molecular Biology on the Cell. (Garland Science, 2002). 33. Daquinag, A. C., Souza, G. R. Kolonin, M. G. Adipose tissue engineering in three-dimensional levitation tissue culture method based on magnetic nanoparticles. Tissue Eng Aspect C Solutions 19, 3364 (2013). 34. Zaman, M. H., Kamm, R. D., Matsudaira, P. Lauffenburger, D. a. Computational model for cell migration in three-dimensional matrices. Biophys J 89, 13897 (2005). 35. Ng, K. W., Leong, D. T. W. Hutmacher, D. W. The challenge to measure cell proliferation in two and 3 dimensions. Tissue Eng 11, 1821 (2005). 36. Monzack, E. L., Gu, X. Masters, K. S. Efficacy of simvastatin treatment of valvular interstitial cells varies together with the extracellular atmosphere. Arterioscler Thromb Vasc Biol 29, 2463 (2009). 37. Seiler, A. E. M. Spielmann, H. The validated embryonic stem cell test to predict embryotoxicity in vitro. Nat Protoc 6, 9618 (2011). 38. Friedrich, J., Seidel, C., Ebner, R. Kunz-Schughart, L. A. Spheroid-based drug screen: MicroRNA Activator supplier considerations and practical strategy. Nat Protoc four, 3094 (2009).AcknowledgementsThis study was funded in part by a National Science Foundation (NSF) Compact Business Innovation Investigation (SBIR) Award Phase I (0945954) and Phase II (1127551) from NSF IIP Division of Industrial Innovation and Partnerships; and also the State of Texas Emerging Technologies Fund (ETF). The authors thank Timothy Quang, Rice University, Houston, TX, with aid in determining the resolution of the mobile device camera apparatus.Author contributionsD.M.T. and J.C. equally contributed in performing the majority of your experiments and analysis in Fig. 3, with important assist from D.S., J.A.G. and W.L.H. within the design and execution of experiments and from S.K.N. and H.T. in data evaluation. M.D., K.P.R., R.M.R., T.C.K. and G.R.S. all contributed for the assay and experimental design. H.T. prepared the manuscript and figures, with enable from T.C.K. and G.R.S.Extra informationSupplementary data accompanies this paper at http://nature/ scientificreports Competing financial interests: The University of Texas M. D. Anderson Cancer Center (UTMDACC) and Rice University, as well as their researchers, have filed patents on the technology and intellectual property reported right here. If licensing or commercialization happens, the researchers are entitled to regular royalties. Glauco R. Souza, Robert M. Raphael, T. C. Killian have equity in Nano3D Biosciences, Inc. UTMDACC and Rice University manage the terms of those arrangements in accordance to their established institutional conflict-of-interest policies. How you can cite this short article: Timm, D.M. et al. A high-throughput three-dim.