s. uniprot.org/uniprot/Q6WUC1 Involved in transport of water and other compact neutral mol ecules across cellular biological membranes (Kapilan et al. 2018) Catalyses the very first committed step of fatty acid synthesis, the carboxylation of acetylCoA to malonylCoA (Sasaki and Nagano 2004) Involved in defensive oleoresin formation in conifers in response to insect attack or other injury. Involved in sesquiterpene (C15) olefins biosynthesis uniprot.org/uniprot/O81086 Involved in gibberellin signal ling (Sun 2011) Mediates the amino acid proline and glycine betaine transport uniprot. org/uniprot/P(2022) 23:NZPradTrx049513_CNZPradTrx049513_CPutative prolinerich arabinoga lactan HIV medchemexpress protein 4e+NZPradTrx079868_CPREDICTED: (RS)norcoclaurine 6OmethyltransferaselikebNZPradTrx054832_CAquaporinlike proteink+NZPradTrx069597_CAcetylCoA carboxylase BCCP subunitaNZPradTrx117954_CEalphabisabolene synthaseb+NZPradTrx087252_CTPA: putative GID1like gibberel lin receptorg+ +(2) Web page 20 ofNZPradTrx074370_CPutative proline transporterkNZPradTrx112166_CNantongo et al. BMC Genomics(2022) 23:Table 5 (continued)Function Related with metal ion binding and oxidoreductase activity uniprot.org/unipr ot/Q9LIG0 T7-B-MJ T7-B-S T7-N-MJ T14-B-MJ T14-B-S T14-N-MJ T21-B-MJ T21-B-S T21-N-MJ +Scion transcript codeGene nameNZPradTrx113904_C06/ NZPradTrx101343_CPREDICTED: clavaminate syn thaselike protein At3g21360 likejaprimary metabolismbsecondary metabolismcdigestive inhibitorsdpathogenesis-related (PR) protein familiesegenes involved with physical strengthening with the cell-wallftranscription factorsgphytohormones and signalling moleculeshgeneral catalystsimolecules involved in transcriptionjmolecules involved in broad biotic and abiotic tension responseskbroad function genesPage 21 ofNantongo et al. BMC Genomics(2022) 23:Web page 22 ofFig. 5 An upset plot displaying the amount of unique and overlapping differentially expressed transcripts following methyl jasmonate (MJ) as well as the bark stripping (strip) treatments more than time and plant components (needles [N] and bark [B]). T7, T14 and T21 referred to sampling undertaken 7, 14 and 21 days after therapy respectively. As an instance, 749 transcripts in the needles (N) have been differentially expressed uniquely at T7 following MJ treatment and had been not differentially expressed at any time point within the bark (B) or other time point inside the needles (N). CXCR3 medchemexpress therapies or occasions exactly where overlapping transcripts occur are linked by lines. For instance, the most widespread overlapping transcripts have been the 227 that have been differentially expressed only inside the needles at T7 and T14 in methyl jasmonate (MJ) remedy. The other transcript combinations are ordered by their frequency of occurrence based on the different exclusive or overlapping combinations in which they were differentially expressed within the methyl jasmonate (MJ) and bark stripping (strip) remedies at each and every time. Note that no transcripts have been differentially expressed inside the needles following bark stripping at any timesignificant down-regulation at T7 in each strip and MJ treated samples.Functional classification of differentially expressed transcriptsTo assess the overall effect in the treatments across unique gene households and molecular processes, the GO terms were determined for the up-regulated and downregulated transcripts for each and every situation (time treatment plant aspect). There was an general similarity inside the GO terms for genes that had been up- and down-regulated within the strip and methyl jasmonate therapies.