Icles. We have recently enhanced the contrast and spatial resolution of SPIRI by pupil function engineering and computational imaging. Methods: In SPIRI, the interference of light reflected from the sensor surface is modified by the presence of particles creating a distinct signal that reveals the size in the particle which is not otherwise visible under a traditional microscope. Using this instrument platform, we’ve got demonstrated label-free identification and visualization of a variety of viruses in multiplexed format in complex samples in a disposable cartridge. Not too long ago, our technology was applied to detection of exosomes and commercialized by Nanoview Biosciences for quantified measurement of exosomes on dry sensor chips. We are at present focusing onISEV2019 ABSTRACT BOOKvarious in-liquid detection too as further improvement in the strategy applying pupil function engineering. Outcomes: By acquiring a number of photos having a partitioned pupil (resulting in structured illumination) and computational imaging, we’ve got demonstrated substantial improvement in visibility of low-index nanoparticles in liquid. Additionally, spatial resolution has been improved beyond the diffraction limit approaching one hundred nm in the visible microscopy. We’ve developed compact and economical sensor chips and microfluidic cartridges permitting for study of biological particles (exosomes and also other extracellular vesicles) directly inside the bodily fluids devoid of labels. Summary/Conclusion: In summary, we’ve got demonstrated enhanced visibility of exosomes in SPIRI utilizing pupil function engineering. Funding: EU-INDEXuse of a number of recognition events in mixture with signal amplification permits detection of exosomes with higher specificity and sensitivity. Summary/Conclusion: Here, we discuss the CD39 Proteins supplier application of proximity assays for sensitive detection of exosomes in physique fluids, to visualize the uptake of exosomes by cells, plus the possible of such method to become used to improved recognize the biology in the exosomes and to determine exosomes as disease biomarkers.OF22.A 96 effectively plate format lipid quantification assay with improved sensitivity for standardization of experiments with extracellular vesicles Tamas Visnovitza, Xabier Osteikoetxeab, Barbara W. S arc, Judith Mihalyd, P er Lrincze, Krisztina V. Vukmana, Eszter nes T ha, Anna Koncza, Inna Sz sf, Robert Horv hf, Zoltan Vargag and Edit I Buz c Semmelweis University, Dept. of Genetics, Cell- and Immunobiology, Budapest, Hungary; bAstraZeneca, Macclesfield, UK; cSemmelweis University, Budapest, Hungary; dRCNS HAS, Budapest, Hungary; e Department of Anatomy, Cell and Adiponectin Proteins custom synthesis Developmental Biology, E v Lor d University, Budapest, Hungary; fNanobiosensorics Laboratory MTA-EKMFA, Budapest, Hungary; gResearch Centre for All-natural Sciences, Hungarian Academy of Sciences, Budapest, HungaryaOF22.Proximity assays for detection and characterization of exosomes Masood Kamali-Moghaddam, Ehsan Manouchehri, Alireza Azimi, Qiujin Shen, Radiosa Gallini and Claudia Fredolini Uppsala University, Uppsala, SwedenIntroduction: Exosomes acquire an elevated interest in simple biology also as in medicine. They’re shown to be involved in quite a few biological processes, and are confirmed to hold terrific potentials as diagnostic and therapeutic tools. Having said that, there’s an unmet want for new and enhanced technologies for quantitative and qualitative characterization of exosomes to meet challenges associated to these vesicles, including low concentrations in physique f.