Ific genes. Final, mass spectrometry of was performed to recognize parasite cargo connected with exosomes from infection. Final results: NTA analysis of plasma-derived exosomes revealed twice as significantly exosomes in sufferers as opposed to healthy donors. Furthermore, in vivo distribution experiments in mice revealed spleen-specific tropism of exosomes from vivax infections when when compared with exosomes from worm infections or controls. Furthermore, a important higher uptake by of exosomes from infections was MMP-14 Proteins Gene ID observed in human spleen fibroblasts when in comparison with exosomes from healthier folks. Noticeably, ICAM-1, a recognized receptor for binding of P. vivax (Bernabeu et al., Cell Microbiol. 2015), was particularly upregulated by exosomes from infections within a dose-dependent manner. Final proteomic analysis of exosomes from infections revealed the presence of parasite proteins linked with them. Summary/Conclusion: These findings suggest that exosomes from natural vivax infections signal human spleen fibroblasts facilitating cytoadherence of the parasite. Funding: This study was funded by Generalitat de Catalunya, Ministerio Espa l de Econom y Competitividad, REDiEX and Fundaci Ram Areces. HT is recipient of an AGAUR PhD fellowship.ISGlobal, Hospital Cl ic Universitat de Barcelona, Barcelona, Spain, Barcelona, Spain; 2Microenvironment and Metastasis Group, Molecular Oncology Plan,Sunday, 06 MayLB02.Alterations in platelet membranes and formation of lipid mediators for the duration of storage Sami Valkonen1; Minna Holopainen2; Jesmond Dalli3; Reijo K el; Pia RM. Siljander1; Saara Laitinen1 EV group, Faculty of Bio-and Environmental Sciences and Faculty of Pharmacy, University of Helsinki, Finland, Helsinki, Finland; 2Finnish Red Cross Blood Service, Helsinki, Finland, Helsinki, Finland; 3William Harvey Analysis Institute, Barts and the London School of Medicine, Queen Mary University of London, London, United kingdom, London, Uk; 4 Division of Physiology and Neuroscience, Department of Biosciences, University of Helsinki,Helsinki, FinlandLB02.Validation of purification techniques for extracellular vesicles Jacopo Zini1; Heikki Saari2; Marjo Yliperttula2; Olli-Petteri NivaroUniversity of Helsinki, Helsinki, Finland; 1University of Helsinki, Division of Pharmaceutical Biosciences, Helsinki, FinlandBackground: The time-dependent variation in glycerophospholipid (GPL) composition of platelets has been studied previously, potentially explaining the proposed hyperlink between the concentrate storage time and incidence of adverse transfusion reactions (ATR). The lipid composition and more particularly the lipid mediator (LM) profile of platelet concentrates is functionally crucial and may perhaps assistance to know the mechanisms of ATR. This know-how may very well be additional exploited to tailor transfusion treatment options to unique kinds of sufferers: because the LM composition modulates immune responses, unique sufferers groups would advantage from platelet concentrates with unique LM profiles. Techniques: Studied samples have been isolated on days 1, 2, 5 and 8 from clinical grade platelet concentrates. The GPL profile with the whole concentrate, platelets and extracellular vesicles (EVs) was analysed with electrospray-ionization mass spectrometry and the content material of LMs in the whole solution and EVs was determined applying Protein tyrosine phosphatases Proteins Source liquid chromatography andem mass spectrometry. Western blot was applied to prove the presence of enzymes creating LMs. Benefits: The GPL profile in the entire concentrate and pl.