E analyzed fat depots in bull carcasses could possibly be on account of smaller variations among the examined production systems, compared with preceding studies. In accordance with the British Department of Well being [30], the n-6/n-3 PUFA ratio ought to not exceed 4.0. Inside the present study, the values on the n-6/n-3 PUFA ratio had been within this variety in all forms of adipose tissue and in both production systems. Bilik et al. [31] identified that distinctive feeding intensity levels in Limousin bulls had a significant effect on the n-6/n-3 PUFA ratio, which was not observed in this study. The proportions of FA groups had been significantly affected by the type of fat, which was also reported by other authors [5,7,32]. Comparable to the present study, Aldai et al. [33] noted that SFA content material was highest in intermuscular fat and lowest in intramuscular fat. High PUFA concentrations in intramuscular fat, compared with other varieties of fat, have been also observed by Harper and Pethick [34], and Pethick et al. [35], who attributed this phenomenon to the little size of adipocytes. As outlined by Nurnberg et al. [36] and Arana et al. [37], a greater ratio of phospholipids to neutral lipids Pyranonigrin A web contributes to a larger proportion of PUFAs in intramuscular fat. The content material of MUFAs in subcutaneous fat was higher inside the present experiment and in prior research [33,38,39]. Bialek and Tokarz [40]Animals 2021, 11,7 ofsuggested that it could result from elevated activity in the enzyme which converts myristic acid (C14:0), palmitic acid (C16:0) and stearic acid (C18:0) to their corresponding MUFAs. In line with Barton et al. [32], a larger proportion of SFAs in perirenal fat, compared with subcutaneous fat, resulted from a larger content material of C18:0. The above authors stressed that a higher proportion of MUFAs in subcutaneous fat, observed also within the present study, might be CL 218872 site linked with larger concentrations of C14:1, C16:1 and C18:1. Stearic acid C18:0 belongs towards the most significant functional FAs. According to Oka et al. [41] and De Smet et al. [42], the percentage of stearic acid decreases in beef carcasses with a rise in their fat content material. This getting was confirmed in the present study exactly where C18:0 content material was reduce inside the fatter carcasses of semi-intensively fattened bulls, and it was lowest in subcutaneous fat. Comparable observations were created by Barton et al. [32]. As stearic acid is very easily converted to oleic acid C18:1, it can be regarded as neutral or capable of decreasing blood cholesterol levels [43]. In accordance with Gebauer et al. [44], myristic acid (C 14:0) and palmitic acid (C 16:0) exert adverse health effects by escalating the threat of cardiovascular illness and cancer. In the existing study, the content of those acids was lowest in intramuscular fat. In the work of Mapiye et al. [45], the proportion of C 14:0 was higher in subcutaneous fat than in perirenal fat. Considerable differences amongst FA groups point for the usually known relationships described in other studies investigating the effects of production systems and diets around the proportion of PUFAs in cattle [1,26,468]. Concentrate-based diets contribute to a greater level of n-6 C18:2 in addition to a lower degree of n-3 C18:3 as well as other long-chain FAs (EPA, DHA and DPA) [49,50], which was also partially confirmed inside the present study. In both this experiment and previous studies [5,7,33,51], CLA content was highest in subcutaneous fat, and its worth determined within this study (0.44) is constant with those reported by the cited authors. In line with Br.