And friction force (FF) images of your laser-patterned DLN film are shown in AdipoRon Biological Activity Figure 10. A region near the corner of the microcrater structure was examinedCoatings 2021, 11,12 ofto evaluate the friction forces on the original and laser-patterned DLN surface. Equivalent towards the prior studies [25], the LFM imaging was carried out employing worn Si ideas with the tip radius of 0.5 . The friction contrast is clearly noticed and characterized by considerably reduce friction forces in the laser-patterned area than on the original surface, see Figure 10b. As a result of somewhat deep craters, the contribution from the surface relief slope for the lateral force signal isn’t completely compensated during subtraction of two lateral force pictures [46], leading to “higher friction” at the crater edges. The lower friction forces within the laser-patterned region are accompanied with a great deal reduce pull-off forces (Fpull-off ) than around the original film, as confirmed by the force istance curves (Figure 11a) measured in distinctive positions inside the FF image in Figure 10b, namely: (1) Fpull-off = 1290 nN on the original film, (2) Fpull-off = 990 nN near the area of redeposited material, (three) Fpull-off = 63 nN in the region of redeposited material, and (4) Fpull-off = 16 nN within the center of a crater. This suggests that the ablated and redeposited material modifications the nanoscale surface properties inside and around the laser-produced microcraters. The location with the low-friction region with redeposited material covers the distance of 102 in the crater edge and, which includes the crater, it covers a circle region of 157 radius. The occurrence on the region “2” with slightly lower friction and pull-off force (than on original Coatings 2021, 11, FOR PEER Evaluation 13 of 16 Coatings 2021, 11, xxFOR PEER Critique 13 of to surface) is most likely caused by mass distribution of ablated clusters/particles, top 16 variation within the structure and/or thickness on the redeposited layer.Figure 10. Surface relief (a) and friction force (b) photos from the laser-patterned DLN film near the corner of a microcrater Figure ten. Surface relief (a) and friction force (b) pictures in the laser-patterned DLN film close to the corner of a microcrater Figure 10. Surface relief (a) and friction force (b) pictures with the laser-patterned DLN film close to the corner of a microcrater structure (shown in Figure 1a), load on Si tip 120 nN. The marked points (1,2,three,4) in the image would be the places of forcestructure (shown in Figure 1a), load onon tiptip 120 nN. The marked points (1,2,three,4) inFFimageimage would be the places of structure (shown in Figure 1a), load Si Si 120 nN. The marked points (1,2,three,4) within the FF FF would be the areas of forcethe distancecurves measurements, shown in Figure 11. curves measurements, shown in Figure 11. distance force istance curves measurements, shown in Figure 11.Figure 11. (a) The force istance curves measured unique points on the DLN film (marked in within the FF image in Figure Figure 11. (a) The force istance curves measured inindifferent points on the DLN film (Ganoderic acid N Technical Information markedthe FF image in Figure 10b): Figure 11. (a) The force istance curves measured in distinctive points around the DLN film (markedin the FF image in Figure 10b): (1) original film, (2) near the area of redeposited material, (3) inside the region of redeposited material, four) within the center 10b): (1) original film, (two) the region of redeposited material, (three) in(3) in the region of redeposited material, 4) in center of a (1) original film, (two) close to close to the regio.