Es secreted by the genus MedChemExpress Eledone peptide Pseudomonas are heatstable. The residual lipase activity in the purified LipM of P. fluorescens isolated from Brazilian raw milk was only . soon after heattreatment of C for s in buffer (Martins et al. It really is noteworthy that Vithanage et al. showed that far more than of Pseudomonas strains isolated from raw milk presented to of residual lipase activity just after a heat treatment of s at C (UHT therapy). Those authors observed far more strains producing heatstable lipases than strains making heatlabile lipases amongst Pseudomonas strain isolated from raw milk. These research confirm that numerous lipases from the genus Pseudomonas can resist heattreatment applied in dairy industries which include pasteurization andor UHT therapy. No heat treatment is accessible that might inactivate these lipases without altering the milk’s sensory and nutritional qualities.Lipase from Bacillus spp. Isolated from Milk and Dairy ProductsThe presence from the thermophilic species Geobacillus stearothermophilus as a lipolytic enzyme producer in raw milk and milk powder has been reported by many authors (Chopra and Mathur,Chen et al. The principal characteristic of Bacillus lipases is the substitution from the very first glycine by alanine in the conserved pentapeptide AXSXG (Arpigny and Jaeger. Most Bacillus lipases show the highest catalytic activities at temperatures ranging from to C (Chen et al. The lipase of Bacillus can be classified in two groups: subfamilies I. and I The lipase of B. subtilis (molecular mass about kDa) may be the smallest true lipase known from bacteria found in raw milk (ArpignyFrontiers in Microbiology www.frontiersin.orgMarch Volume ArticleMachado et al.Spoilage Microbiota in Dairy Productsand Jaeger. This lipase,belonging to subfamily I can also be secreted by B. licheniformis,frequently encountered in raw milk as a lipolytic enzyme producer (Baur et al a). Using a molecular mass of kDa,the lipase secreted by the species G. thermocatenulatus and G. stearothermophilus belongs to subfamily I. and shows optimal activity at pH . and C (SchmidtDannert et al. Kim et al. Arpigny and Jaeger. As described above for Pseudomonas,a lot of Bacillus (or Geobacillus) lipases remain stable in the course of heattreatments utilized in dairy industries (pasteurization andor UHT treatment) and can for that reason affect milk and dairy goods through storage. Chen et al. calculated a t of min at C in buffer at pH . for the lipase developed by a strain of G. stearothermophilus. Thinking about all lipases secreted by Bacillus,lipases from strains isolated from milk powder production presented a larger residual activity just after pasteurization at C for min in milk (Chen et al. Furthermore,a current study showed that a lot more than of Bacillus strains isolated from raw milk presented to of residual lipase activity just after a heat treatment at C for s (Vithanage et al.Phospholipase CThe production of distinct phospholipases has been reported for Gramnegative and Grampositive psychrotrophs (S haug and Stepaniak. The phospholipase most studied is phospholipase C,which may be either hemolytic or nonhemolytic. Phospholipase C activity has been detected in the genera Pseudomonas,Bacillus,Serratia,Hafnia,Acinetobacter,and Microbacterium in raw milk by Vithanage et al. . However,in accordance with De Jonghe et al. ,within the genus Bacillus only the species B. cereus is capable to generate the phospholipase C. The presence of this enzyme was not observed for PubMed ID:https://www.ncbi.nlm.nih.gov/pubmed/20972551 the species B. licheniformis or B. subtilis. This enzyme is par.