8) 0.0 (0.0?0.0) 1.0 (0.3?1.5) 0.8 (0.2?1.3) 0.0 (0.0?0.0) 1.3 (0.6?2.0) 0.9 (0.3?1.4) 0.0 (0.0?0.0) 1.1 (0.4?1.7) 1.1 (0.4?1.7) 0.0 (0.0?0.0) 0.5 (0.1?0.8) (Continued)+ + + + + + + + + + + + + + + -163 17.8 (15.3?0.2) 08 02 04 46 00 01 0.9 (0.2?.4) 0.2 (0.1?.5) 0.4 (0.1?.8) 5.0 (3.6?.4) 0.0 (0.0?.0) 0.1 (0.1?.3)372 40.6 (37.4?3.7) 02 01 21 07 00 99 01 00 10 04 00 56 32 00 01 0.2 (0.1?.5) 0.1 (0.1?.3) 2.3 (1.3?.2) 0.8 (0.2?.3) 0.0 (0.0?.0) 10.8 (8.8?2.8) 0.1 (0.1?.3) 0.0 (0.0?.0) 1.1 (0.4?.7) 0.4 (0.1?.8) 0.0 (0.0?.0) 6.1 (4.5?.6) 3.5 (2.3?.6) 0.0 (0.0?.0) 0.1 (0.1?.3)PLOS ONE | DOI:10.1371/journal.pone.0159037 July 19,6 /Clustering of Risk Factors in Dalfopristin chemical information AdolescentsTable 3. (Continued) Risk Factors 1 Physical inactivity + 0 Excessive alcohol consumption + Smoking Sedentary behaviour + Unhealthy diet + n Prevalence Observed (95 CI) 09 16 00 01 05 02 1.0 (0.3?.6) 1.8 (0.9?.6) 0.0 (0.0?.0) 0.1 (0.1?.3) 0.6 (0.1?.0) 0.2 (0.08?.5) Expected (95 CI) 1.6 (0.7?.4) 1.0 (0.3?.5) 0.1 (0.1?.1) 0.1 (0.1?.2) 0.5 (0.1?.9) 0.1 (0.1?.3) O/E (95 CI) 0.6 (0.1?1.0) 1.8 (1.0?2.6) 0.0 (0.0?0.0) 1.6 (0.8?2.3) 1.2 (0.5?1.8) 1.6 (0.8?2.3)+ -CI: Confidence interval; + presence of risk behavior; bsence of risk behavior; O: Observed prevalence; E: Expected prevalence; O/E: Ratio between observed and expected prevalence. doi:10.1371/journal.pone.0159037.tAssociations between the dependent variable “clustering of risk factors” and both demographic and economic variables were analysed by multinomial logistic regression, with estimations of the odds ratio (OR) and respective confidence intervals (CI 95 ). The reference category was “no risk factor”; however, due to the low frequency of individuals in this category (0.2 ), it was combined with the category “one risk factor” (3.7 ). Therefore, “none or one risk factor” was considered the reference (3.9 ). The adjusted analysis controlled for all independent variables of the study (sex, age, skin colour, school shift and economic level) (Table 4). Mdivi-1 biological activity Moreover, binary logistic regression was performed for simultaneous risk factors that had a higher prevalence in this population (6.0 of adolescents had both physical inactivity and unhealthy diet, 10.8 of adolescents had sedentary lifestyle and unhealthy diet, 40.5 had physical inactivity, sedentary behaviour and unhealthy diet, and 17.7 had physical inactivity, excessive alcohol consumption, sedentary behaviour and unhealthy diet), along with the estimated OR and respective 95 confidence interval. The adjusted analysis controlled for all independent variables of the study (sex, age, skin colour, school shift and economic level) (Table 5). We adopted p < 0.05 as the level of significance for all statistical tests, and we used Stata113.0 (College Station, Texas, USA) and SPSS1 21.0 (Armonk, New York, USA).ResultsOverall, 916 adolescents aged 16.1 ?1.1 years were analysed. Most subjects were female (55.4 ), aged 14?6 years (59.2 ), of white skin colour (63.1 ), students of the day shift (73.7 ) and of a high economic level (69.5 ). About eight in ten adolescents did not perform physical activity on a regular basis (77.3 ) and had sedentary behaviour equal to or greater than four hours (87.4 ). One third of adolescents used alcohol excessively (33.7 ), and one in 10 adolescents smoked cigarettes (7.9 ). The consumption of unhealthy food was observed in 92.1 of adolescents (Table 2). Female adolescents had a higher prevalence of physical inactivity (81.3 ) than male adolesc.8) 0.0 (0.0?0.0) 1.0 (0.3?1.5) 0.8 (0.2?1.3) 0.0 (0.0?0.0) 1.3 (0.6?2.0) 0.9 (0.3?1.4) 0.0 (0.0?0.0) 1.1 (0.4?1.7) 1.1 (0.4?1.7) 0.0 (0.0?0.0) 0.5 (0.1?0.8) (Continued)+ + + + + + + + + + + + + + + -163 17.8 (15.3?0.2) 08 02 04 46 00 01 0.9 (0.2?.4) 0.2 (0.1?.5) 0.4 (0.1?.8) 5.0 (3.6?.4) 0.0 (0.0?.0) 0.1 (0.1?.3)372 40.6 (37.4?3.7) 02 01 21 07 00 99 01 00 10 04 00 56 32 00 01 0.2 (0.1?.5) 0.1 (0.1?.3) 2.3 (1.3?.2) 0.8 (0.2?.3) 0.0 (0.0?.0) 10.8 (8.8?2.8) 0.1 (0.1?.3) 0.0 (0.0?.0) 1.1 (0.4?.7) 0.4 (0.1?.8) 0.0 (0.0?.0) 6.1 (4.5?.6) 3.5 (2.3?.6) 0.0 (0.0?.0) 0.1 (0.1?.3)PLOS ONE | DOI:10.1371/journal.pone.0159037 July 19,6 /Clustering of Risk Factors in AdolescentsTable 3. (Continued) Risk Factors 1 Physical inactivity + 0 Excessive alcohol consumption + Smoking Sedentary behaviour + Unhealthy diet + n Prevalence Observed (95 CI) 09 16 00 01 05 02 1.0 (0.3?.6) 1.8 (0.9?.6) 0.0 (0.0?.0) 0.1 (0.1?.3) 0.6 (0.1?.0) 0.2 (0.08?.5) Expected (95 CI) 1.6 (0.7?.4) 1.0 (0.3?.5) 0.1 (0.1?.1) 0.1 (0.1?.2) 0.5 (0.1?.9) 0.1 (0.1?.3) O/E (95 CI) 0.6 (0.1?1.0) 1.8 (1.0?2.6) 0.0 (0.0?0.0) 1.6 (0.8?2.3) 1.2 (0.5?1.8) 1.6 (0.8?2.3)+ -CI: Confidence interval; + presence of risk behavior; bsence of risk behavior; O: Observed prevalence; E: Expected prevalence; O/E: Ratio between observed and expected prevalence. doi:10.1371/journal.pone.0159037.tAssociations between the dependent variable "clustering of risk factors" and both demographic and economic variables were analysed by multinomial logistic regression, with estimations of the odds ratio (OR) and respective confidence intervals (CI 95 ). The reference category was "no risk factor"; however, due to the low frequency of individuals in this category (0.2 ), it was combined with the category "one risk factor" (3.7 ). Therefore, "none or one risk factor" was considered the reference (3.9 ). The adjusted analysis controlled for all independent variables of the study (sex, age, skin colour, school shift and economic level) (Table 4). Moreover, binary logistic regression was performed for simultaneous risk factors that had a higher prevalence in this population (6.0 of adolescents had both physical inactivity and unhealthy diet, 10.8 of adolescents had sedentary lifestyle and unhealthy diet, 40.5 had physical inactivity, sedentary behaviour and unhealthy diet, and 17.7 had physical inactivity, excessive alcohol consumption, sedentary behaviour and unhealthy diet), along with the estimated OR and respective 95 confidence interval. The adjusted analysis controlled for all independent variables of the study (sex, age, skin colour, school shift and economic level) (Table 5). We adopted p < 0.05 as the level of significance for all statistical tests, and we used Stata113.0 (College Station, Texas, USA) and SPSS1 21.0 (Armonk, New York, USA).ResultsOverall, 916 adolescents aged 16.1 ?1.1 years were analysed. Most subjects were female (55.4 ), aged 14?6 years (59.2 ), of white skin colour (63.1 ), students of the day shift (73.7 ) and of a high economic level (69.5 ). About eight in ten adolescents did not perform physical activity on a regular basis (77.3 ) and had sedentary behaviour equal to or greater than four hours (87.4 ). One third of adolescents used alcohol excessively (33.7 ), and one in 10 adolescents smoked cigarettes (7.9 ). The consumption of unhealthy food was observed in 92.1 of adolescents (Table 2). Female adolescents had a higher prevalence of physical inactivity (81.3 ) than male adolesc.