Using agarose gel electrophoresis analysis. Amp-resistant and Km-resistant transformants were cured
Using agarose gel electrophoresis analysis. Amp-resistant and Km-resistant transformants were cured of pMD20-oriR and I-BRD9 pUCK-repB by subculturing in LB broth without Amp and Km. The absence of pO157 was…