Curacy in detailed fibrosis classification may 
provide by liver biopsy; however, this method does has innate limits, such as invasive, sampling error and sample size effect, which limits its application. Consequently, the highly specific set of biomarkers for fibrosis grading always pursue by professionals.In the past two decades, a number of markers in combination with clinical risk Lixisenatide biological activity factors are used to evaluate fibrosis grade, such as FibroMeter, FibroTest, etc [5,6], all being used for diagnosing significant fibrosis. In fact, the combination of several MedChemExpress tert-Butylhydroquinone biomarker for evaluating fibrosis grading were better prognosis predictors than histological staging [7,8]. Those methods have one common point, i.e., consisting of several serum marker and mathematical model used to calculate fibrosis index. Since all of those noninvasive models have moderate accuracy for determining significant fibrosis, new biomarkers for the diagnosis of significant fibrosis are still in strong demand by clinicians. Recently, a novel Golgi protein, GP73, was used to diagnosing hepatocelullar carcinoma [9]. This protein initially reported by Kladney RD, et al [10], and they also found that GP73 expression is increased in cultured cells by viruses infection [10], Subsequently, several investigations demonstrated that GP73 protein is overexpressed in a variety of acute and chronic liver diseases [11], and serum concentration correlated with progression of chronic liver disease [12,13]. However, the relationship between serum GP73 1662274 concentration and staging or grading of chronic liver disease is still a pendent question. The present study was designed to evaluate the serum GP73 for diagnosing significant fibrosis and liver cirrhosis.GP73, a Marker for Evaluating HBV ProgressionTable 1. Patient’s clinical characteristic.(Hotgen Biotech Inc., Beijing, China), according to the manufacturer’s protocol.Parameter Group Sex/age Male Female Clinical diagnosis Chronic hepatitis Liver cirrhsis Comorbidity Diabetes HypertensionDescription FibroScan (761) liver biopsy 23727046 (633)Transient elastography measurementLiver stiffness was measured with a FibroScanH device (FibroScanH, Philips, France), based on manufacturer’s protocol. Results were expressed in kilopascals (kPa). Ten successful acquisitions were performed for each patient, and the median value was calculated by the device. The cut-off point of liver stiffness score for significant fibrosis, and liver cirrhosis referenced to the previous report [14,15] i.e. 8.8 kPa(F2), for significant fibrosis, 16.9 kPa for liver cirrhosis.492 (39.48612.32 yrs) 269 (40.62613.76 yrs)440(36.11610.72 yrs) 193(34.07610.37 yrs)*649 (85.28 ) 112 (14.72 )582(91.94 ) 51(8.06 )Liver biopsy and Immunohistochemistry48(6.31 ) 11(1.45 ) 17(2.69 ) 7(1.11 ) 2(0.32 )Coronary heart disease 4(0.53 ) HBeAg Positive Negative HBV DNA , 2 log 2 log BMI (Kg/m2) Male(mean 6 S.D.) Female(mean 6 S.D.) Total bilirubin (mmol/L) Albumin (g/L) Prothrombin time (s) 22.6065.12 20.7364.93* 22.79638.0 43.3666.11 12.8862.20 68(8.94 ) 693(91.06 ) 436(57.29 ) 325(42.71 )397(62.72 ) 236(37.28 )36(5.69 ) 597(94.31 )24.2666.67 21.3363.91* 21.30637.54 42.8666.06 12.7169.Liver biopsies were obtained using 16G disposable needles (Hepafix, Germany). Fibrosis staging was considered reliable when the liver specimen length was 1.5 cm or the portal tract number 10. Liver specimens were stained with Masson trichrome and interpreted by two highly experienced liver pathologists. Liver fibro.Curacy in detailed fibrosis classification may provide by liver biopsy; however, this method does has innate limits, such as invasive, sampling error and sample size effect, which limits its application. Consequently, the highly specific set of biomarkers for fibrosis grading always pursue by professionals.In the past two decades, a number of markers in combination with clinical risk factors are used to evaluate fibrosis grade, such as FibroMeter, FibroTest, etc [5,6], all being used for diagnosing significant fibrosis. In fact, the combination of several biomarker for evaluating fibrosis grading were better prognosis predictors than histological staging [7,8]. Those methods have one common point, i.e., consisting of several serum marker and mathematical model used to calculate fibrosis index. Since all of those noninvasive models have moderate accuracy for determining significant fibrosis, new biomarkers for the diagnosis of significant fibrosis are still in strong demand by clinicians. Recently, a novel Golgi protein, GP73, was used to diagnosing hepatocelullar carcinoma [9]. This protein initially reported by Kladney RD, et al [10], and they also found that GP73 expression is increased in cultured cells by viruses infection [10], Subsequently, several investigations demonstrated that GP73 protein is overexpressed in a variety of acute and chronic liver diseases [11], and serum concentration correlated with progression of chronic liver disease [12,13]. However, the relationship between serum GP73 1662274 concentration and staging or grading of chronic liver disease is still a pendent question. The present study was designed to evaluate the serum GP73 for diagnosing significant fibrosis and liver cirrhosis.GP73, a Marker for Evaluating HBV ProgressionTable 1. Patient’s clinical characteristic.(Hotgen Biotech Inc., Beijing, China), according to the manufacturer’s protocol.Parameter Group Sex/age Male Female Clinical diagnosis Chronic hepatitis Liver cirrhsis Comorbidity Diabetes HypertensionDescription FibroScan (761) liver biopsy 23727046 (633)Transient elastography measurementLiver stiffness was measured with a FibroScanH device (FibroScanH, Philips, France), based on manufacturer’s protocol. Results were expressed in kilopascals (kPa). Ten successful acquisitions were performed for each patient, and the median value was calculated by the device. The cut-off point of liver stiffness score for significant fibrosis, and liver cirrhosis referenced to the previous report [14,15] i.e. 8.8 kPa(F2), for significant fibrosis, 16.9 
kPa for liver cirrhosis.492 (39.48612.32 yrs) 269 (40.62613.76 yrs)440(36.11610.72 yrs) 193(34.07610.37 yrs)*649 (85.28 ) 112 (14.72 )582(91.94 ) 51(8.06 )Liver biopsy and Immunohistochemistry48(6.31 ) 11(1.45 ) 17(2.69 ) 7(1.11 ) 2(0.32 )Coronary heart disease 4(0.53 ) HBeAg Positive Negative HBV DNA , 2 log 2 log BMI (Kg/m2) Male(mean 6 S.D.) Female(mean 6 S.D.) Total bilirubin (mmol/L) Albumin (g/L) Prothrombin time (s) 22.6065.12 20.7364.93* 22.79638.0 43.3666.11 12.8862.20 68(8.94 ) 693(91.06 ) 436(57.29 ) 325(42.71 )397(62.72 ) 236(37.28 )36(5.69 ) 597(94.31 )24.2666.67 21.3363.91* 21.30637.54 42.8666.06 12.7169.Liver biopsies were obtained using 16G disposable needles (Hepafix, Germany). Fibrosis staging was considered reliable when the liver specimen length was 1.5 cm or the portal tract number 10. Liver specimens were stained with Masson trichrome and interpreted by two highly experienced liver pathologists. Liver fibro.