Bands have been detected by incubation with a secondary antibody conjugated to horseradish peroxidase and chemiluminescence substrate (ECL Furthermore, GE Healthcare, Pittsburgh, PA, United states)
The unfavorable control in each and every team was incubated only with the secondary antibody and DAPI. Anti-atubulin (Sigma), tetramethylrhodamine-labeled phalloidin (Lifestyle technologies, Foster City, CA, United states of america),…