ml DAPI staining remedy (.1% TritonX a hundred in ten ml PBS, a hundred ml of one mg/ml DAPI) and analyzed by FACS
The cells were being seeded in triplicate in 96 nicely plates at 36103 cells/effectively. Immediately after 24, forty eight, and 72 hrs, 10 ml of WST-one reagent (Roche, Indianapolis, IN)…